Investigation of changes in serum thiols and neutrophil-to-lymphocyte ratio, monocyte-to-lymphocyte ratio, platelet-to-lymphocyte ratio, and mean platelet volume/platelet count ratio indices in cats undergoing ovariohysterectomy

Abstract

The aim of this study was to investigate complete blood indices [neutrophil-to-lymphocyte ratio (NLR), monocyte-to-lymphocyte ratio (MLR), platelet-to-lymphocyte ratio (PLR) and mean platelet volume/platelet count ratio (MPV/PLT)] and thiols (native and thiol) in cats undergoing ovariohysterectomy (OHE). The study sample comprised a total of 10 female cats of mixed breeds. Ovariohysterectomy operation was performed under Ketamine/ Xylazine anesthesia with the appropriate technique. Blood samples were collected from the cephalic vein pre-OHE and post-OHE at 2 h, 24 h, and 7 d. Total and native thiol levels in the serum were analyzed using a colorimetric technique, and whole blood analysis was carried out using anticoagulant tubes. White Blood Cell (WBC) and neutrophil increased significantly at 2 h post-OHE, peaked at 24 h post-OHE, then returned to baseline levels by 7 d (P<0.001). The lymphocyte and thiols decreased at 2 h, 24 h, and 7 d post-OHE, and the lowest lymphocyte count was observed at 24 h (P<0.001). The lowest monocyte count was observed on d 7 post-OHE (P<0.001). NLR increased significantly 2 h after OHE, peaked at 24 h after OHE, and remained high on d 7 (P<0.001). MLR increased at 2 h, peaked at 24 h, then returned to baseline levels on d 7 (P<0.001). PLR increased gradually and peaked at 24 h and on d 7 (P<0.001). MPV/PLT after OHE was statistically lowest on d 7 (P<0.05). A negative correlation was determined between NLR, MLR, PLR, and thiols (P<0.001). In conclusion, thiols and complete blood indices (NLR, MLR, and PLR) may be important in the assessment of inflammation and stress responses after OHE in cats.